The ATP-dependent RNA helicase HrpB plays an important role in motility and biofilm formation in Xanthomonas citri subsp. citri
Identifieur interne : 000039 ( Main/Exploration ); précédent : 000038; suivant : 000040The ATP-dependent RNA helicase HrpB plays an important role in motility and biofilm formation in Xanthomonas citri subsp. citri
Auteurs : Laís Moreira Granato ; Simone Cristina Picchi ; Maxuel De Oliveira Andrade [États-Unis] ; Marco Aurélio Takita ; Alessandra Alves De Souza ; Nian Wang [États-Unis] ; Marcos Antonio MachadoSource :
- BMC Microbiology [ 1471-2180 ] ; 2016.
Abstract
RNA helicases are enzymes that catalyze the separation of double-stranded RNA (dsRNA) using the free energy of ATP binding and hydrolysis. DEAD/DEAH families participate in many different aspects of RNA metabolism, including RNA synthesis, RNA folding, RNA-RNA interactions, RNA localization and RNA degradation. Several important bacterial DEAD/DEAH-box RNA helicases have been extensively studied. In this study, we characterize the ATP-dependent RNA helicase encoded by the
The
Our data show that
The online version of this article (doi:10.1186/s12866-016-0655-1) contains supplementary material, which is available to authorized users.
Url:
DOI: 10.1186/s12866-016-0655-1
PubMed: 27005008
PubMed Central: 4804567
Affiliations:
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">The ATP-dependent RNA helicase HrpB plays an important role in motility and biofilm formation in <italic>Xanthomonas citri</italic> subsp. <italic>citri</italic></title><author><name sortKey="Granato, Lais Moreira" sort="Granato, Lais Moreira" uniqKey="Granato L" first="Laís Moreira" last="Granato">Laís Moreira Granato</name><affiliation><nlm:aff id="Aff1">Centro de Citricultura Sylvio Moreira/IAC, Rodovia Anhanguera Km 158, Cordeirópolis, SP 13490-970 Brazil</nlm:aff><wicri:noCountry code="subfield">SP 13490-970 Brazil</wicri:noCountry></affiliation><affiliation><nlm:aff id="Aff2">Universidade Estadual de Campinas/UNICAMP, Instituto de Biologia, P.O. Box 6010, Campinas, SP 13083-970 Brazil</nlm:aff><wicri:noCountry code="subfield">SP 13083-970 Brazil</wicri:noCountry></affiliation></author><author><name sortKey="Picchi, Simone Cristina" sort="Picchi, Simone Cristina" uniqKey="Picchi S" first="Simone Cristina" last="Picchi">Simone Cristina Picchi</name><affiliation><nlm:aff id="Aff1">Centro de Citricultura Sylvio Moreira/IAC, Rodovia Anhanguera Km 158, Cordeirópolis, SP 13490-970 Brazil</nlm:aff><wicri:noCountry code="subfield">SP 13490-970 Brazil</wicri:noCountry></affiliation></author><author><name sortKey="Andrade, Maxuel De Oliveira" sort="Andrade, Maxuel De Oliveira" uniqKey="Andrade M" first="Maxuel De Oliveira" last="Andrade">Maxuel De Oliveira Andrade</name><affiliation wicri:level="2"><nlm:aff id="Aff3">Citrus Research and Educational Center, Department of Microbiology and Cell Science, University of Florida, IFAS, 700 Experiment Station Road, Lake Alfred, FL 33850 USA</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Floride</region></placeName><wicri:cityArea>Citrus Research and Educational Center, Department of Microbiology and Cell Science, University of Florida, IFAS, 700 Experiment Station Road, Lake Alfred</wicri:cityArea></affiliation></author><author><name sortKey="Takita, Marco Aurelio" sort="Takita, Marco Aurelio" uniqKey="Takita M" first="Marco Aurélio" last="Takita">Marco Aurélio Takita</name><affiliation><nlm:aff id="Aff1">Centro de Citricultura Sylvio Moreira/IAC, Rodovia Anhanguera Km 158, Cordeirópolis, SP 13490-970 Brazil</nlm:aff><wicri:noCountry code="subfield">SP 13490-970 Brazil</wicri:noCountry></affiliation></author><author><name sortKey="De Souza, Alessandra Alves" sort="De Souza, Alessandra Alves" uniqKey="De Souza A" first="Alessandra Alves" last="De Souza">Alessandra Alves De Souza</name><affiliation><nlm:aff id="Aff1">Centro de Citricultura Sylvio Moreira/IAC, Rodovia Anhanguera Km 158, Cordeirópolis, SP 13490-970 Brazil</nlm:aff><wicri:noCountry code="subfield">SP 13490-970 Brazil</wicri:noCountry></affiliation></author><author><name sortKey="Wang, Nian" sort="Wang, Nian" uniqKey="Wang N" first="Nian" last="Wang">Nian Wang</name><affiliation wicri:level="2"><nlm:aff id="Aff3">Citrus Research and Educational Center, Department of Microbiology and Cell Science, University of Florida, IFAS, 700 Experiment Station Road, Lake Alfred, FL 33850 USA</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Floride</region></placeName><wicri:cityArea>Citrus Research and Educational Center, Department of Microbiology and Cell Science, University of Florida, IFAS, 700 Experiment Station Road, Lake Alfred</wicri:cityArea></affiliation></author><author><name sortKey="Machado, Marcos Antonio" sort="Machado, Marcos Antonio" uniqKey="Machado M" first="Marcos Antonio" last="Machado">Marcos Antonio Machado</name><affiliation><nlm:aff id="Aff1">Centro de Citricultura Sylvio Moreira/IAC, Rodovia Anhanguera Km 158, Cordeirópolis, SP 13490-970 Brazil</nlm:aff><wicri:noCountry code="subfield">SP 13490-970 Brazil</wicri:noCountry></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">27005008</idno><idno type="pmc">4804567</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4804567</idno><idno type="RBID">PMC:4804567</idno><idno type="doi">10.1186/s12866-016-0655-1</idno><date when="2016">2016</date><idno type="wicri:Area/Pmc/Corpus">000613</idno><idno type="wicri:Area/Pmc/Curation">000612</idno><idno type="wicri:Area/Pmc/Checkpoint">000028</idno><idno type="wicri:Area/Ncbi/Merge">001F71</idno><idno type="wicri:Area/Ncbi/Curation">001F71</idno><idno type="wicri:Area/Ncbi/Checkpoint">001F71</idno><idno type="wicri:Area/Main/Merge">000039</idno><idno type="wicri:Area/Main/Curation">000039</idno><idno type="wicri:Area/Main/Exploration">000039</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">The ATP-dependent RNA helicase HrpB plays an important role in motility and biofilm formation in <italic>Xanthomonas citri</italic> subsp. <italic>citri</italic></title><author><name sortKey="Granato, Lais Moreira" sort="Granato, Lais Moreira" uniqKey="Granato L" first="Laís Moreira" last="Granato">Laís Moreira Granato</name><affiliation><nlm:aff id="Aff1">Centro de Citricultura Sylvio Moreira/IAC, Rodovia Anhanguera Km 158, Cordeirópolis, SP 13490-970 Brazil</nlm:aff><wicri:noCountry code="subfield">SP 13490-970 Brazil</wicri:noCountry></affiliation><affiliation><nlm:aff id="Aff2">Universidade Estadual de Campinas/UNICAMP, Instituto de Biologia, P.O. Box 6010, Campinas, SP 13083-970 Brazil</nlm:aff><wicri:noCountry code="subfield">SP 13083-970 Brazil</wicri:noCountry></affiliation></author><author><name sortKey="Picchi, Simone Cristina" sort="Picchi, Simone Cristina" uniqKey="Picchi S" first="Simone Cristina" last="Picchi">Simone Cristina Picchi</name><affiliation><nlm:aff id="Aff1">Centro de Citricultura Sylvio Moreira/IAC, Rodovia Anhanguera Km 158, Cordeirópolis, SP 13490-970 Brazil</nlm:aff><wicri:noCountry code="subfield">SP 13490-970 Brazil</wicri:noCountry></affiliation></author><author><name sortKey="Andrade, Maxuel De Oliveira" sort="Andrade, Maxuel De Oliveira" uniqKey="Andrade M" first="Maxuel De Oliveira" last="Andrade">Maxuel De Oliveira Andrade</name><affiliation wicri:level="2"><nlm:aff id="Aff3">Citrus Research and Educational Center, Department of Microbiology and Cell Science, University of Florida, IFAS, 700 Experiment Station Road, Lake Alfred, FL 33850 USA</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Floride</region></placeName><wicri:cityArea>Citrus Research and Educational Center, Department of Microbiology and Cell Science, University of Florida, IFAS, 700 Experiment Station Road, Lake Alfred</wicri:cityArea></affiliation></author><author><name sortKey="Takita, Marco Aurelio" sort="Takita, Marco Aurelio" uniqKey="Takita M" first="Marco Aurélio" last="Takita">Marco Aurélio Takita</name><affiliation><nlm:aff id="Aff1">Centro de Citricultura Sylvio Moreira/IAC, Rodovia Anhanguera Km 158, Cordeirópolis, SP 13490-970 Brazil</nlm:aff><wicri:noCountry code="subfield">SP 13490-970 Brazil</wicri:noCountry></affiliation></author><author><name sortKey="De Souza, Alessandra Alves" sort="De Souza, Alessandra Alves" uniqKey="De Souza A" first="Alessandra Alves" last="De Souza">Alessandra Alves De Souza</name><affiliation><nlm:aff id="Aff1">Centro de Citricultura Sylvio Moreira/IAC, Rodovia Anhanguera Km 158, Cordeirópolis, SP 13490-970 Brazil</nlm:aff><wicri:noCountry code="subfield">SP 13490-970 Brazil</wicri:noCountry></affiliation></author><author><name sortKey="Wang, Nian" sort="Wang, Nian" uniqKey="Wang N" first="Nian" last="Wang">Nian Wang</name><affiliation wicri:level="2"><nlm:aff id="Aff3">Citrus Research and Educational Center, Department of Microbiology and Cell Science, University of Florida, IFAS, 700 Experiment Station Road, Lake Alfred, FL 33850 USA</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Floride</region></placeName><wicri:cityArea>Citrus Research and Educational Center, Department of Microbiology and Cell Science, University of Florida, IFAS, 700 Experiment Station Road, Lake Alfred</wicri:cityArea></affiliation></author><author><name sortKey="Machado, Marcos Antonio" sort="Machado, Marcos Antonio" uniqKey="Machado M" first="Marcos Antonio" last="Machado">Marcos Antonio Machado</name><affiliation><nlm:aff id="Aff1">Centro de Citricultura Sylvio Moreira/IAC, Rodovia Anhanguera Km 158, Cordeirópolis, SP 13490-970 Brazil</nlm:aff><wicri:noCountry code="subfield">SP 13490-970 Brazil</wicri:noCountry></affiliation></author></analytic><series><title level="j">BMC Microbiology</title><idno type="eISSN">1471-2180</idno><imprint><date when="2016">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background</title><p>RNA helicases are enzymes that catalyze the separation of double-stranded RNA (dsRNA) using the free energy of ATP binding and hydrolysis. DEAD/DEAH families participate in many different aspects of RNA metabolism, including RNA synthesis, RNA folding, RNA-RNA interactions, RNA localization and RNA degradation. Several important bacterial DEAD/DEAH-box RNA helicases have been extensively studied. In this study, we characterize the ATP-dependent RNA helicase encoded by the <italic>hrpB</italic> (XAC0293) gene using deletion and genetic complementation assays. We provide insights into the function of the <italic>hrpB</italic> gene in <italic>Xanthomonas citri</italic> subsp. <italic>citri</italic> by investigating the roles of <italic>hrpB</italic> in biofilm formation on abiotic surfaces and host leaves, cell motility, host virulence of the citrus canker bacterium and growth <italic>in planta</italic>.</p></sec><sec><title>Results</title><p>The <italic>hrpB</italic> gene is highly conserved in the sequenced strains of <italic>Xanthomonas</italic>. Mutation of the <italic>hrpB</italic> gene (<italic>∆hrpB</italic>) resulted in a significant reduction in biofilms on abiotic surfaces and host leaves. <italic>∆hrpB</italic> also exhibited increased cell dispersion on solid medium plates. <italic>∆hrpB</italic> showed reduced adhesion on biotic and abiotic surfaces and delayed development in disease symptoms when sprayed on susceptible citrus leaves. Quantitative reverse transcription-PCR assays indicated that deletion of <italic>hrpB</italic> reduced the expression of four type IV pili genes. The transcriptional start site of <italic>fimA</italic> (XAC3241) was determined using rapid amplification of 5′-cDNA Ends (5′RACE). Based on the results of <italic>fimA</italic> mRNA structure predictions, the <italic>fimA</italic> 5′ UTR may contain three different loops. HrpB may be involved in alterations to the structure of <italic>fimA</italic> mRNA that promote the stability of <italic>fimA</italic> RNA.</p></sec><sec><title>Conclusions</title><p>Our data show that <italic>hrpB</italic> is involved in adherence of <italic>Xanthomonas citri</italic> subsp. <italic>citri</italic> to different surfaces. In addition, to the best of our knowledge, this is the first time that a DEAH RNA helicase has been implicated in the regulation of type IV pili in <italic>Xanthomonas</italic>.</p></sec><sec><title>Electronic supplementary material</title><p>The online version of this article (doi:10.1186/s12866-016-0655-1) contains supplementary material, which is available to authorized users.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Silverman, E" uniqKey="Silverman E">E Silverman</name></author><author><name sortKey="Edwalds Gilbert, G" uniqKey="Edwalds Gilbert G">G Edwalds-Gilbert</name></author><author><name sortKey="Lin, Rj" uniqKey="Lin R">RJ Lin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cordin, O" uniqKey="Cordin O">O Cordin</name></author><author><name sortKey="Banroques, J" uniqKey="Banroques J">J Banroques</name></author><author><name sortKey="Tanner, Nk" uniqKey="Tanner N">NK 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Machado</name><name sortKey="Picchi, Simone Cristina" sort="Picchi, Simone Cristina" uniqKey="Picchi S" first="Simone Cristina" last="Picchi">Simone Cristina Picchi</name><name sortKey="Takita, Marco Aurelio" sort="Takita, Marco Aurelio" uniqKey="Takita M" first="Marco Aurélio" last="Takita">Marco Aurélio Takita</name></noCountry><country name="États-Unis"><region name="Floride"><name sortKey="Andrade, Maxuel De Oliveira" sort="Andrade, Maxuel De Oliveira" uniqKey="Andrade M" first="Maxuel De Oliveira" last="Andrade">Maxuel De Oliveira Andrade</name></region><name sortKey="Wang, Nian" sort="Wang, Nian" uniqKey="Wang N" first="Nian" last="Wang">Nian Wang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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